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Recorded On:5/12/2022
Duration:60 minutes
Dr. Guozheng Shao and Dr. Anna Scott discuss the development of an osmotic membrane for use in a portable dialysis device and its validation using amino acid analysis to quantify urea removal using this novel membrane. Dr. Guozheng Shao: Dialysate Regeneration with Urea-Selective Membrane Coupled to Photoelectrochemical Oxidation System
Portable dialysis can meaningfully improve the health outcomes and life quality of end-stage renal disease patients and reduce the economic burdens of dialysis-related care. Current treatment sessions use 120 Kg of aqueous dialysate, thus key to realizing portable dialysis is the continuous regeneration of small volumes of spent dialysate. Urea is the single most abundant uremic waste. However, its removal is compromised due to the interference of other uremic toxins and nutrients. Dr. Guozheng Shao discusses the development of an osmotic membrane which demonstrates high selectivity of urea over other uremic species in both reverse osmosis and in forward osmosis (FO) modes. By using a second stage dialyzer with a commercially available forward osmosis cartridge, daily urea removal rate of over 30 g, twice the daily generation rate, could be achieved. Combined with an optimized urea photodecomposition device, selective removal of urea and excessive water were achieved simultaneously, enabling the dialyzer to meet clinical needs. Dr. Anna Scott: Free Amino Acid Quantification - Use in Clinical Testing and Research Collaborations
Quantification of free amino acids in different bodily fluids is essential for supporting and managing a variety of inborn errors of metabolism. Dr. Anna Scott provides an overview of the test for Dr. Shao’s research and its value in the hospital setting. She also describes the expertise of Seattle Children's biochemical genetics laboratory in free amino acid quantification, their ability to support development studies of a novel dialysis device, and collaboration for studies that need reproducible quantification of metabolites. Key topics include:
- How uremic toxins are quantified during a typical hemodialysis session
- Methods of urea removal for a portable dialysis device
- Demonstrating a forward osmosis membrane with high selectivity and high flux for urea removal
- Achieving efficient urea photooxidation with a randomly-oriented highly crystalline TiO2 nanowire substrate
- How this membrane could serve as a barrier to increase the urea removal efficiency and safety of a portable dialysis device
- Describe genetic disorders for which doctors use amino acid analysis as a supplement for diagnosis
- Identify two methods used to measure free amino acids
- Identify at least three assay parameters routinely tracked as part of assay maintenance
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Speakers
 Guozheng Shao, PhD Acting Instructor, Division of Nephrology University of Washington Dr. Guozheng Shao is currently an Acting Instructor in the Division of Nephrology, Department of Medicine at the University of Washington, Seattle. In the past few years, Dr. Shao has been focusing on developing a portable dialysis device. Dr. Shao’s work has won a $650,000 prize from KidneyX in 2021. He was an Amazon Catalyst Fellow in 2016.
Dr. Shao received his PhD from the Department of Chemistry at the University of Washington, Seattle in 2014. He received Bachelor’s degree from Peking University in 2008.  Anna Scott, PhD Director, Biochemical Genetics Laboratory Seattle Children's Hospital Anna Scott, PhD, FACMG, is Director of the Biochemical Genetics laboratory at Seattle Children's Hospital. She completed her graduate work in biochemistry and structural biology at the University of California, Davis. After graduate school, she completed a clinical biochemical genetics fellowship at Stanford University. As a clinical assistant professor, Dr. Scott is interested in inborn errors of metabolism and mitochondrial diseases with a focus on changes in enzyme activity. Her team closely partners with Washington state's newborn screening program to support diagnostic testing for infants with abnormal newborn screens.
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