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Approaches to Studying Neuronal Signaling in the Brain of Awake Behaving Mice

清醒活动小鼠神经元活性及神经信号的研究方法

Live date  直播日期:12/14/2021

duration  时长:60 minutes / 60分钟


Recording neuronal activity in awake behaving mice is indispensable for understanding sensory processing, cognition, or decision making.  But there’s the rub: the targeted brain region must remain stable relative to the optical or electrical probe. This task is not trivial, because an awake mouse wants to move and explore its environment. Besides that: some experimental techniques may cause stress to awake mice thus undoing the benefits of removing anesthetics and the biases associated with them. Are we doomed to always choose between the two evils: anesthesia or stress?

The newest approaches to recording neuronal activity in awake mice seek to avoid both of these culprits.  They roughly fall into one of the two categories: head-fixed and head-mounted approaches. This presentation describes both types of approaches and their representative commercial solutions. Besides that: it helps to understand the pros and cons of various solutions from the standpoint of different research objectives and methods. The presentation highlights the newest commercial head-fixation solution, the Mobile HomeCage, which allows correlating neural recordings with 2D locomotion and complex behavior, such as social interactions or maze navigation (in real tangible mazes).  

在多种大脑研究的方法学中,使用清醒活动小鼠作为模型对于研究感觉、认知或决策制定等是必不可少的。但在实验过程中,目标大脑区域必须相对于显微镜或电子探针保持极为稳定的状态。但要做到这一点很具有挑战性,因为清醒的动物会移动并想要探索周边环境。但在另一方面,如果不进行麻醉,一些实验手段可能会使清醒的小鼠产生较大的压力,从而使结果出现偏差。至此为止,我们总要在麻醉和实验压力这两种干扰因素中做出取舍。

近年来一些最新的方法学不断试图降低和避免这两种在清醒小鼠神经研究中的干扰因素。它们大致可分为两大类:头部固定式和头戴式方案。本讲座主要介绍了这两类方案及其代表性的常见方法学,同时我们将从不同研究对象和方法学的角度讨论各种方案的利弊。该讲座重点介绍了最新一代的头部固定方案,气浮笼(英文名:Mobile HomeCage),它将神经信号与2D运动,复杂行为学关联在一起,例如社交互动或真实可触摸式迷宫导航等。

Key learning objectives  主要内容

  • Anesthesia and stress both distort neuronal recordings. Fortunately, it is no longer necessary to choose between two evils
  • Differences between two novel approaches to correlating neuronal activity with behavior, head-mounted vs. head-fixed preparations
  • The pros and cons of the most prominent commercial solutions within each category
  • The Mobile HomeCage is the most recent/advanced head-fixation solution that allows integration with 2D locomotion, and complex behavior (sociability, maze navigation in real, tangible mazes)

  • 研究清醒的动物大脑比研究麻醉大脑更为接近实际情形
  • 头戴式与头固定式作为两种新的方案提供了在清醒动物中观察神经信号的研究平台
  • 常见清醒动物大脑研究方案的特点和各自的应用领域
  •  气浮笼是最先进的头部固定方案之一,并将神经信号与2D运动,复杂行为学关联在一起,例如社交互动或真实可触摸迷宫等


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Speaker  主讲人

Freddy Yan 闫旭

Application Specialist 应用专家

Neurotar

闫旭神经学博士毕业于芬兰赫尔辛基大学。在Dr.Henri Huttunen团队做大学研究员期间,Dr.闫旭致力于开发高通量筛选针对神经退行性疾病靶点药物的平台。2019-2021年,Dr.闫旭在中国葛兰素史克担任过资深医学联络官以及医学事务副经理。他目前在芬兰Neurotar公司就职,担任应用专家,并致力于支持世界各地的实验室和代理合作伙伴,为将清醒小鼠神经信号与行为学研究联系起来提供最佳的解决方案。

Dr. Freddy Yan received his Ph.D. in Neuroscience from Helsinki University, Finland. During his tenure as a researcher in Dr. Henri Huttunen’s group, Dr.Yan developed a drug discovery platform for the high throughput screening of compounds targeting neurodegenerative diseases. In 2019-2021, Dr. Yan worked as a senior medical scientific liaison and medical affairs associate manager at GlaxoSmithKline, China. He is currently employed at Neurotar as an application specialist. In this role, Dr. Yan is supporting labs and distribution partners all over the world in their quest for an optimal solution for correlating neuronal signaling with behavior in awake mice. Dr.
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